Reference genes for QRT-PCR tested under various stress conditions in Folsomia candida and Orchesella cincta (Insecta, Collembola)
نویسندگان
چکیده
منابع مشابه
3. Reference genes for RT-qPCR tested under various stress conditions in Folsomia candida and Orchesella cincta (Insecta, Collembola)
Genomic studies measuring transcriptional responses to changing environments and stress currently make their way into the field of evolutionary ecology and ecotoxicology. To investigate a small to medium number of genes or to confirm large scale microarray studies, Quantitative Reverse Transcriptase PCR (RT-qPCR) can achieve high accuracy of quantification when key standards, such as normalizat...
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The collembolan, Folsomia candida, is widely used in soil ecotoxicology. In recent years, growth rate of collembolans has become as frequently used endpoint as reproduction rate in ecotoxicological studies. However, measuring collembolan body sizes to estimate growth rate is a complicated and time-consuming task. Here we present a new image analysis method, which facilitates and accelerates the...
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Genetic variation for thermal plasticity plays an important role in the success or failure of a species with respect to the colonization of different thermal habitats and the ability to deal with climatic change. The aim of this paper is to study the relative contribution of the additive and non-additive components of genetic variation for the slope of the temperature reaction norm for juvenile...
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BACKGROUND Quantitative real-time reverse-transcriptase PCR (qRT-PCR) is an important technique for analyzing differences in gene expression due to its sensitivity, accuracy and specificity. However, the stability of the expression of reference genes is necessary to ensure accurate qRT-PCR assessment of expression in genes of interest. Perennial ryegrass (Lolium perenne L.) is important forage ...
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Quantitative RT-PCR can be a very sensitive and powerful technique for measuring differential gene expression. Changes in gene expression induced by abiotic stresses are complex and multifaceted, which make determining stably expressed genes for data normalization difficult. To identify the most suitable reference genes for abiotic stress studies in soybean, 13 candidate genes collected from li...
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ژورنال
عنوان ژورنال: BMC Molecular Biology
سال: 2009
ISSN: 1471-2199
DOI: 10.1186/1471-2199-10-54